This collagen-based scaffold provides your cells a physiologically friendly growth environment. Tantti BioScaffold can be degraded by trypsin in five minutes to easily recover your valuable cells. The feature is particularly suitable for drug screening, cancer research, precision medicine, and regenerative medicine.
What is 3D cell culture?
In 1980s, Dr. Mina Bissell is the first to develop the three-dimensional (3D) cell culture technology in breast cancer research at Lawrence Berkeley National Laboratory, USA. The 3D cell culture technology is to provide constructs for mammalian’s cell attachment and proliferation, migration and differentiation. Natural polymers such as collagen, cellulose, glycosaminoglycan and synthetic materials such as polystyrene (PS), DEAE-dextran are the main materials for 3D bio-scaffolds. The advantages of natural polymer-based bio-scaffold are biocompatibility, low toxicity and immune response-free, otherwise the synthetic material-based bio-scaffold has good mechanical properties and reproducibility. The interactions between cell-cell and cell-extracellular matrix (ECM) are simulated through 3D cell culture scaffold. Therefore, the 3D scaffold is a potential technology to be applied to regenerative medicine, tissue engineering, cell therapy and biomedical science instead of the traditional 2D cell culture.
Applications of 3D bio-scaffold in biomedical science
The 3D bio-scaffold plays an important role in tissue engineering, oncology, regenerative medicine, drug screening and stem cell biology study. To engineer an artificial organ, cells express similar behaviors, such as cell proliferation, ECM production, cell-cell interaction and cell-ECM interaction with those are in vivo through a 3D bio-scaffold. For drug screening, to avoid the efficacy-prediction error and side effects, an appropriate model is necessary for drug evaluation. Furthermore, the 3D scaffolds as an in vitro model are also used for drug evaluation and long-term clinical effects.
Introduction of Tantti® 3D Cell Culture scaffold
Tantti® 3D Cell Culture scaffold is manufactured from medical grade gelatin with excellent mechanical properties and thermal stability. The unique 3D Cell Culture scaffold with micrometer pore size, more surface area and highly connectivity is designed by the pioneer microfluid perforating technology licensed by Academia Sinica exclusively. The special porosity structure allows fully gas exchange and nutrient metabolism upregulating the cell viability and proliferation. Importantly, Tantti® 3D Cell Culture scaffold is dissolvable by using a specific enzyme and the cell recovery rate reaches to 100%. Otherwise, the pore size, thickness and mechanical properties are adjustable to meet customer needs.
Springerplus. 2014 Feb 11;3:80. doi: 10.1186/2193-1801-3-80.
Bioactive Materials. Volume 3, Issue 3, September 2018, Pages 278-314