What is a liquid chromatography column?
With the advancement of biotechnology, biopharmaceuticals have flourished in recent years, and the trend would greatly drive the growth of the analysis and downstream purification market of the pharmaceutical industry. In the market for analysis and purification, for the application of macromolecular drugs, the faster and more effective liquid chromatography column has become an important issue of the industry.
Liquid chromatography columns were used in a wide range of applications, including water purification, biopharmaceuticals, food ingredient analysis. The report ,"Case study on high-efficiency chromatography materials and application in biomedical separation and purification" point out that downstream separation and purification account about 50 to 80% cost of biopharmaceuticals, which is often the bottleneck of biopharmaceutical production, and with more increasing challenges with the biopharmaceutical separation and purification process, latest biotechnology applications are being applied. The innovation of chromatography materials and manufacturing method will bring new application opportunities for the liquid chromatography systems.
Applications of a monolithic column on liquid chromatography.
Traditional chromatography column is packed with uniform micro or nanospheres in the column so that when mobile phase and the target are flowing in the gap between the spheres (the stationary phase), the different interaction between the two phases will result in differences in residence time in the column. The disadvantage of the conventional chromatography column is too small gap between the balls, and the moving phase will cause very large backpressure, so the flow rate will be very slow. Therefore, the monolithic column is a very useful purification method in the downstream separation process. The monolithic column is different from the traditional packing column. The monolithic column has a whole piece of the connected substrate, and the internal random distributed channel is bigger than the traditional column, because of the larger channel, it will greatly improve operation speed and maintain lower backpressure, constant efficiency of analysis and purification. By some studies had shown that overall column purification speed is more than twice of the conventional column, and the purification performance can keep very consistent under high flow rate. It is especially suitable for protein, monoclonal antibody, and virus particles purification in biopharmaceuticals, this reason is monolithic column can offer large channels to allow macromolecule to pass quickly and maintaining excellent separation purpose at the same time.
Introduction of Tantti® Monolith
Tantti Laboratory Inc. can synthesize a variety of uniform micro to nanoscale polymer spheres with different particle size and surface functionalization. By the powerful patented technologies, Tantti can make large volume column, with flat or cylindrical structures, and provide material and surface modification service according to market demand. Tantti was proud of the innovative technology and created a series of products, such as size-exclusion, ion-exchange, hydrophilicity, and affinity monolithic column. This highly connected honeycomb structure is suitable for the small-scale rapid analysis and large-scale purification of biopharmaceuticals in the international market.