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Tantti Microcarrier

The Tantti microcarrier is a unique porous microporous microcarrier with high connectivity, uniform pores, and high surface area. It is manufactured from medical grade gelatin with high mechanical strength and thermal stability. When cells are cultured in the microcarrier, the high surface area provides more adhesion area for the cells and the large pore structure allows the cells to adhere to the external and internal surface. The microcarriers internal structure can serve as protection from shear stress-induced damages for the cells growing inside. The interconnected pores ensure that the growth environment of the cells inside the microcarrier is uniformly stabilized, increasing the activity and productivity of the cells. In addition, when harvesting the cells, the microcarriers can be dissolved with trypsin, and the recovery of viable cells can be almost 100%.
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Large pore structure
Large pore structure
High surface area
High surface area
Dissolvable
Dissolvable
Uniform pores
Uniform pores
High connectivity
High connectivity

What is Microcarrier?

 

Microcarrier provides a 3D construct for anchorage-dependent cells attachment and proliferation published by van Wezel group in Nature in 1967. It recommended that 3D microcarrier culture is able to replace 2D monolayer culture for cell expansion. Microcarriers are made of either natural materials, such as gelatin, collagen and cellulose or synthetic materials, such as polystyrene(PS), polyhydroxyethylmethacrylate(pHEMA) and DEAE-dextran. Natural materials or its derivates with better biocompatibility, softer mechanical properties and higher reproducibility variation than synthetic materials. In addition, lacking binding sites or functional groups of synthetic materials for cell adhesion is one of the major issues for cell expansion. Therefore, natural materials-based microcarrier becomes more popular recently.

 

Microcarrier Applications in Bio-Medical Science

 

Cell number expansion is the first priority in tissue engineering, cell therapy and vaccine manufacturing. Although the 2D cell culture is easy to apply for cell expansion, the issues such as time consuming, labor intensive and the unexpectable cell behaviors need to be defined. Therefore, microcarrier which is providing 3D culture platform and more surface area for cell adhesion and proliferation is able to reach the ideal cell number for tissue engineering, cell therapy and vaccine manufacturing.

 

 

Introduction of Tantti® Dissolvable Microcarriers

 

Tantti® microcarrier is a unique macroporous construct with highly connectivity uniform pores and numerous surface area (6000 cm2/g). It is manufactured by medical-grade gelatin providing flexible mechanical properties and stability. The highly connective and uniform pores allow cells to adhere, proliferate, migrate and differentiate and the nutrients, oxygens and metabolic waste exchange effectively from the external to the internal microcarrier. The macroporous microcarrier as a house-like structure to cells for protecting cells from shear stress exposure. In addition, the microcarrier is dissolved easily by specific enzymes and the cell recovery rate is almost 100%.

 

 

Validation

 

 

 

Relevant Article

 

Biotechnol J. 2016 Mar;11(4):473-86. doi: 10.1002/biot.201400862. Epub 2016 Feb 29.

Tissue Engineering and Regenerative Medicine June 2016, Volume 13, Issue 3, pp 235–241

 

 

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Tantti Microcarrier