What is Microcarrier?
Microcarrier provides a 3D construct for anchorage-dependent cells attachment and proliferation published by van Wezel group in Nature in 1967. It recommended that 3D microcarrier culture is able to replace 2D monolayer culture for cell expansion. Microcarriers are made of either natural materials, such as gelatin, collagen and cellulose or synthetic materials, such as polystyrene(PS), polyhydroxyethylmethacrylate(pHEMA) and DEAE-dextran. Natural materials or its derivates with better biocompatibility, softer mechanical properties and higher reproducibility variation than synthetic materials. In addition, lacking binding sites or functional groups of synthetic materials for cell adhesion is one of the major issues for cell expansion. Therefore, natural materials-based microcarrier becomes more popular recently.
Microcarrier Applications in Bio-Medical Science
Cell number expansion is the first priority in tissue engineering, cell therapy and vaccine manufacturing. Although the 2D cell culture is easy to apply for cell expansion, the issues such as time consuming, labor intensive and the unexpectable cell behaviors need to be defined. Therefore, microcarrier which is providing 3D culture platform and more surface area for cell adhesion and proliferation is able to reach the ideal cell number for tissue engineering, cell therapy and vaccine manufacturing.
Introduction of Tantti® Dissolvable Microcarriers
Tantti® microcarrier is a unique macroporous construct with highly connectivity uniform pores and numerous surface area (6000 cm2/g). It is manufactured by medical-grade gelatin providing flexible mechanical properties and stability. The highly connective and uniform pores allow cells to adhere, proliferate, migrate and differentiate and the nutrients, oxygens and metabolic waste exchange effectively from the external to the internal microcarrier. The macroporous microcarrier as a house-like structure to cells for protecting cells from shear stress exposure. In addition, the microcarrier is dissolved easily by specific enzymes and the cell recovery rate is almost 100%.
Biotechnol J. 2016 Mar;11(4):473-86. doi: 10.1002/biot.201400862. Epub 2016 Feb 29.
Tissue Engineering and Regenerative Medicine June 2016, Volume 13, Issue 3, pp 235–241