Tantti® Monolith is a monolithic column product made from a hyperporous polymer material designed for downstream separation and purification of large biomolecules – particularly in the production of biologic products (e.g., protein drugs, vaccines, and antibodies). Tantti® Monolith's key differentiating characteristic, in comparison to traditional purification columns, is its highly connected honeycomb structure that is produced using Tantti's patented manufacturing process. This structure confers superior porosity, higher flow rates, and lower back pressure when purifying large biomolecules at high loads. This represents a breakthrough in purification technology with the potential to dramatically enhance purification performance while reducing costs for our customers in both small-scale laboratory settings as well as large-scale industrial production.
Cell-Based and Biologic Therapies Driving Need for Greater Efficiency in the Downstream Separation and Purification of Biomolecules
The fast-growing usage of cell-based and biologic therapeutics as well as the trend towards larger targeted biomolecules has driven a need for improvement in biomolecule production efficiency. Given that a critical bottleneck in the production process is in the purification and separation of targeted biomolecules, this has created a need for innovation in purification column technology.
Traditional liquid chromatography columns have consisted of micro- or nanospheres (beads), typically polymer-based, packed closely together in a column through which a liquid containing a target molecule is passed. While this type of column performs well for separating smaller biomolecules, it is less efficient in separating and purifying larger biomolecules (e.g., viral particles) due to the less surface area and incompatible pore structure. Monolithic columns, in contrast, consist of a single piece of a connected substrate, that contains a well-controlled and consistent interpore size (1-3 μm) as well as highly interconnected channels that together achieve a higher binding capacity and hydraulic permeability (>2x in some studies). Relative to traditional columns, monolithic columns exhibit lower backpressure as well as more consistent efficiency, even at high loads. For these reasons, monolithic columns are especially suitable for the purification of nucleic acids and viral particles that are used in biopharmaceutical therapies.
1 Case study on high-efficiency chromatography materials and application in biomedical separation and purification
Tantti® Monolith uses a globally-patented In-Mold Polymerization (IMP) method to produce a monolithic column with highly uniform porosity. Our method results in a highly connected honeycomb structure which differs from the traditional packed-beads design used in the downstream bio-purification. Our IMP technology can also be used to adsorb and remove various trace substances, such as endotoxins, circulating tumor DNA, DNA impurities in the vaccine production process, and host cell protein (HCP).
In addition to controlling porosity, Tantti can also provide material and surface modification as required by the customer to modify parameters such as ion-exchange, hydrophobicity, and affinity.